Inchaço na Organóides intestinal induzida por forscolina: An<em> In Vitro</em> Ensaio para a Avaliação de Medicamentos Response na Fibrose Cística Os pacientes
This protocol describes an assay for measuring CFTR function and CFTR modulator responses in cultured tissue from subjects with cystic fibrosis (CF). Biopsy-derived intestinal organoids swell in a cAMP-driven fashion, a response that is defective (or strongly reduced) in CF organoids and can be restored by exposure to CFTR modulators.