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Swab Sampling Method for the Detection of Human Norovirus on Surfaces
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Immunology and Infection
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JoVE Journal Immunology and Infection
Swab Sampling Method for the Detection of Human Norovirus on Surfaces

Swab Sampling Method for the Detection of Human Norovirus on Surfaces

DOI:
10.3791/55205-v

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10:03 min

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February 06, 2017

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, ,
1Division of Viral Diseases,Centers for Disease Control and Prevention

Chapters

  • 00:05Title
  • 01:04Swab Sampling, Virus Concentration, Viral RNA Extraction, and Purification
  • 02:36Midi Column Viral Nucleic Acid Extraction
  • 03:40Viral Nucleic Acid Concentration
  • 05:16Multiplex RT-qPCR Detection
  • 07:09Quantification of Norovirus in Swab Samples and Hemi-nested Conventional PCR Amplification
  • 08:11Results: Macrofoam-based Swab Sampling Method Effectively Detects Norovirus Outbreaks
  • 09:24Conclusion

Summary

Automatic Translation

Automatic Translation

A macrofoam based sampling methodology was developed and evaluated for the detection and quantification of norovirus on environmental hard surfaces.

Tags

Keywords: Swab SamplingNorovirusEnvironmental SurfaceMacrofoamSample CollectionSample StorageSample TransportLaboratory ProtocolSurface ContaminationRemediation StrategiesStainless SteelToilet SeatDoorknobUNEX BufferPBSTColiphage MS2Lysis SolutionEthanolMidi ColumnNucleic Acid Extraction

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