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泄殖腔按摩与精子 DNA 分离法采集禽精
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Biology
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JoVE Journal Biology
Avian Semen Collection by Cloacal Massage and Isolation of DNA from Sperm
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泄殖腔按摩与精子 DNA 分离法采集禽精

DOI:
10.3791/55324-v

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07:40 min

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February 05, 2018

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,
1Environmental and Conservation Sciences Program,North Dakota State University, 2Department of Biological Sciences,North Dakota State University

Chapters

  • 00:04Title
  • 00:41Semen Collection from a Passerine Bird Using Cloacal Massage
  • 03:34Extraction of DNA from Bird Semen
  • 06:32Results: Success by Bird Species and DNA Electrophoresis
  • 07:08Conclusion

Summary

Automatic Translation

Automatic Translation

本协议描述泄殖腔按摩, 一种非侵入性的手工技术, 用于从禽类中收集精子, 以及使用商业提取试剂盒进行 DNA 提取, 并进行修改。

Tags

Avian Semen CollectionCloacal MassageDNA IsolationSpermBehavioral EcologyEvolutionary EcologySperm CompetitionEpigenetic InheritanceField CollectionCaptive BirdsBreeding SeasonCloaca EversionSemen ExtractionMicrohematocrit TubeSperm VisualizationSample Storage

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