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Modelización del comportamiento del cáncer de ovario en un esferoide multicelular Microdevice 3D dinámico peritoneal
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1School of Biological Sciences,University of Hong Kong, 2Department of Mechanical Engineering,University of Hong Kong, 3Department of Medicine,University of Hong Kong, 4Department of Biomedical Sciences, Key Laboratory of Biochip Technology, Biotech and Health Centre,Shenzhen Research Institutes of City University of Hong Kong
Chapters
- 00:05Title
- 00:49Microfluidic Device Master Design
- 02:32Polydimethylsiloxane Chip Fabrication
- 04:26Seeding the Microfluidic Chip with Primary Peritoneal Mesothelial Cells
- 06:08Ovarian Cancer Spheroid Formation and Co-culture
- 08:09Perfusion Platform Assembly and Co-culture
- 09:16Results: Ovarian Cancer Spheroid Behavior in a 3D Peritoneal Microfluidic Device
- 10:09Conclusion
Para estudiar la progresión del tumor de ovario en un modelo fisiológicamente relevante, esferoides multicelulares se cultivaron en un microdispositivo bajo un flujo de fluido simulado. Este modelo 3D dinámico emula el medio ambiente intraperitoneal con los componentes celulares y mecánicas donde se produce la metástasis del cáncer de ovario.
