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在直肠癌原位小鼠模型中循环肿瘤细胞的分离
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JoVE Journal Cancer Research
Isolation of Circulating Tumor Cells in an Orthotopic Mouse Model of Colorectal Cancer
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在直肠癌原位小鼠模型中循环肿瘤细胞的分离

DOI:
10.3791/55357-v

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06:19 min

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July 18, 2017

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, , , , , ,
1Department of Gastrointestinal, Thoracic and Vascular Surgery, Medizinische Fakultät Carl Gustav Carus,Technische Universität Dresden, 2German Cancer Consortium (DKTK), 3German Cancer Research Center (DKFZ)

Chapters

  • 00:05Title
  • 00:40Orthotopic Mouse Model
  • 03:05Circulating Tumor Cell (CTC) Isolation from Whole Blood
  • 04:59Results: Representative Murine Colorectal Cancer Induction
  • 05:33Conclusion

Summary

Automatic Translation

Automatic Translation

我们描述了通过将肿瘤细胞或有机体注入小鼠盲肠并随后从该模型中分离循环肿瘤细胞（CTC）来建立原位结肠直肠肿瘤。

Tags

Circulating Tumor CellsOrthotopic Mouse ModelColorectal CancerMicrosurgical TechniqueCecal Wall InjectionReproducible Metastatic TumorsImmunocompromised MouseIntra-cecal InjectionMicro Injection PumpBinocular Surgical MicroscopeDensity Gradient MediumBlood Sample Collection

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