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Un protocole pour Rapid Post-mortem Culture cellulaire de Diffuse Intrinsic Pontine gliome (DIPG)
This content is Free Access.
1Graduate Program in Neuroscience, Department of Neurology, Institute for Stem Cell Biology and Regenerative Medicine,Stanford University School of Medicine, 2Departments of Neurology, Neurosurgery, Pathology and Pediatrics, Institute for Stem Cell Biology and Regenerative Medicine,Stanford University School of Medicine
Chapters
- 00:05Title
- 01:12Mechanical Dissociation
- 03:25Enzymatic Dissociation
- 04:50Sucrose Gradient Centrifugation
- 05:49ACK Red Blood Cell Lysis and Initial Culture Maintenance
- 06:50Results: Images of Primary Cultures and Early-passage Cells
- 07:41Conclusion
Ce protocole décrit une méthode pour le traitement rapide de la post-mortem diffuse des échantillons de gliome pontique intrinsèques pour l'établissement de modèles de culture de cellules provenant de patients ou la caractérisation directe des cellules tumorales et microenvironnement.
