Journal
/
Neuroscience
/
Analyzing Dendritic Morphology in Columns and Layers
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Neuroscience
Analyzing Dendritic Morphology in Columns and Layers

Analyzing Dendritic Morphology in Columns and Layers

DOI:
10.3791/55410-v

•

08:41 min

•

March 23, 2017

•

, , , , ,
1Section on Neuronal Connectivity, Eunice Kennedy Shriver National Institute of Child Health and Human Development,National Institutes of Health (NIH), 2Mathematical and Statistical Computing Laboratory, Center for Information Technology,National Institutes of Health (NIH), 3Biomedical Imaging Research Services Section, Center for Information Technology,National Institutes of Health (NIH)

Chapters

  • 00:05Title
  • 00:44Dual-image Acquisition
  • 03:11Neurite Tracing and Reference Point Assignment
  • 06:49Results: Comparison of Tm20 and Tm2 Neuron Morphology
  • 08:04Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we show how to analyze dendritic routing of Drosophila medulla neurons in columns and layers. The workflow includes a dual-view imaging technique to improve the image quality and computational tools for tracing, registering dendritic arbors to the reference column array and for analyzing the dendritic structures in 3D space.

Tags

Keywords: Dendritic MorphologyDrosophila Medulla NeuronsColumnsLayersNeuroscienceDendritic WiringImagingConfocal MicroscopyGFP LabelingBrain ClearingSample Preparation

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/55410?article_type=v

Automated Sholl Analysis of Digitized Neuronal Morphology at Multiple Scales
check_url/55410?article_type=v

Analysis of Dendritic Spine Morphology in Cultured CNS Neurons
check_url/55410?article_type=v

Inducing Dendritic Growth in Cultured Sympathetic Neurons
check_url/55410?article_type=v

The Analysis of Purkinje Cell Dendritic Morphology in Organotypic Slice Cultures
check_url/55410?article_type=v

Visualizing the Effects of a Positive Early Experience, Tactile Stimulation, on Dendritic Morphology and Synaptic Connectivity with Golgi-Cox Staining
check_url/55410?article_type=v

Fast Micro-iontophoresis of Glutamate and GABA: A Useful Tool to Investigate Synaptic Integration
check_url/55410?article_type=v

The Indirect Neuron-astrocyte Coculture Assay: An In Vitro Set-up for the Detailed Investigation of Neuron-glia Interactions
check_url/55410?article_type=v

Quantitative Analysis of Neuronal Dendritic Arborization Complexity in Drosophila
check_url/55410?article_type=v

Visualizing and Analyzing Intracellular Transport of Organelles and Other Cargos in Astrocytes
check_url/55410?article_type=v

Analyzing Mitochondrial Transport and Morphology in Human Induced Pluripotent Stem Cell-Derived Neurons in Hereditary Spastic Paraplegia

Read Article