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Cellular Redox Profiling ved hjælp af højindholdsmikroskopi
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Cellular Redox Profiling ved hjælp af højindholdsmikroskopi

Article DOI: 10.3791/55449-v • 11:37 min • May 14th, 2017
May 14th, 2017 •
1,2, 1,2, 1, 3, 1,2
1Laboratory of Cell Biology and Histology, Department of Veterinary Sciences, University of Antwerp, 2Cell Systems and Imaging Research Group (CSI), Department of Molecular Biotechnology, Ghent University, 3Department of Biochemistry , Radboud Institute for Molecular Life Sciences, Radboud University Medical Center

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I dette papir præsenteres en mikroskopisk arbejdsstrøm med høj indhold til samtidig kvantificering af intracellulære ROS-niveauer samt mitokondrielt membranpotentiale og morfologi - i fællesskab betegnet mitokondriel morfofunktion - i levende adhærente celler ved anvendelse af de cellemeorante fluorescerende reportermolekyler 5- (og- 6) -chloromethyl-2 ', 7'-dichlorodihydrofluoresceindiacetat, acetylester (CM-H2 DCFDA) og tetramethylrhodaminmethylester (TMRM).

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Cellular Biology Reactive Oxygen Species Mitochondria Mitochondrial Morphofunction Live Cell Imaging Højindholdsmikroskopi Fluorescensmikroskopi Quantitative Multiparametric Microscopy Redox Biology
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