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使用高含量显微镜的细胞氧化还原分析
JoVE Journal
Biology
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JoVE Journal Biology
Cellular Redox Profiling Using High-content Microscopy
DOI:

11:37 min

May 14, 2017

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Chapters

  • 00:05Title
  • 00:47Seeding Cells in a 96-well Plate
  • 01:48Setting Up the Microscope and Acquisition Protocol
  • 03:10Loading of the Cells with CM-H2DCFDA and TMRM
  • 04:18First and Second Live Imaging Rounds
  • 06:22Image Processing and Analysis
  • 09:35Results: Effect of Saquinavir on Primary Human Fibroblasts
  • 10:38Conclusion

Summary

Automatic Translation

本文提出了一种高含量显微镜工作流程,用于同时定量细胞内ROS水平,以及线粒体膜电位和形态 - 共同称为线粒体形态功能 - 使用细胞透过性荧光报告分子5-(和 - 6) - 氯甲基-2',7'-二氯二氢荧光素二乙酸酯,乙酰酯(CM-H 2 DCFDA)和四甲基罗丹明甲酯(TMRM)。

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