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JoVE Journal
Biology

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使用高含量显微镜的细胞氧化还原分析
 
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使用高含量显微镜的细胞氧化还原分析

Article DOI: 10.3791/55449-v 11:37 min May 14th, 2017
May 14th, 2017

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Summary

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Click here for the English version.

本文提出了一种高含量显微镜工作流程,用于同时定量细胞内ROS水平,以及线粒体膜电位和形态 - 共同称为线粒体形态功能 - 使用细胞透过性荧光报告分子5-(和 - 6) - 氯甲基-2',7'-二氯二氢荧光素二乙酸酯,乙酰酯(CM-H 2 DCFDA)和四甲基罗丹明甲酯(TMRM)。

Tags

细胞生物学,第123期,活性氧物质,线粒体,线粒体形态,活细胞成像,高含量显微镜,荧光显微镜,定量多参数显微镜,氧化还原生物学
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