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Profilatura cellulare Redox usando la microscopia ad alto contenuto
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Profilatura cellulare Redox usando la microscopia ad alto contenuto

Article DOI: 10.3791/55449-v • 11:37 min • May 14th, 2017
May 14th, 2017 •
1,2, 1,2, 1, 3, 1,2
1Laboratory of Cell Biology and Histology, Department of Veterinary Sciences, University of Antwerp, 2Cell Systems and Imaging Research Group (CSI), Department of Molecular Biotechnology, Ghent University, 3Department of Biochemistry , Radboud Institute for Molecular Life Sciences, Radboud University Medical Center

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Questo documento presenta un flusso di lavoro di microscopia ad alto contenuto per la quantificazione simultanea dei livelli ROS intracellulari, nonché il potenziale e la morfologia della membrana mitocondriale - definiti congiuntamente come morfofunzione mitocondriale - nelle cellule adesive viventi utilizzando le molecole reporter fluorescenti per cellule permeanti 5- 6) clorometil-2 ', 7'-diclorodidro-fluoresceina diacetato, acetil estere (CM-H 2 DCFDA) e tetrametilrodamina metilestere (TMRM).

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Biologia cellulare numero 123 specie di ossigeno reattivo mitocondri morfofunzione mitocondriale imaging a cellule vive microscopia ad alto contenuto microscopia a fluorescenza microscopia quantitativa multiparametrica Redox Biology
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