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Neuroscience
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使用荧光探针定量培养的神经元中的内体和溶酶体运动
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Quantification of Endosome and Lysosome Motilities in Cultured Neurons Using Fluorescent Probes
Please note that all translations are automatically generated.
Click here for the English version.
使用荧光探针定量培养的神经元中的内体和溶酶体运动
DOI:
10.3791/55488-v
•
08:15 min
•
May 22, 2017
•
Fuminori Tsuruta
2,3
,
Tomomi Okajima
,
Sarasa Yano
,
Tomoki Chiba
2,3
1
Graduate School of Life and Environmental Sciences
,
University of Tsukuba
,
2
PhD Program in Human Biology, School of Integrative and Global Majors
,
University of Tsukuba
,
3
Life Science Center of Tsukuba Advanced Research Alliance (TARA)
,
University of Tsukuba
Chapters
00:05
Title
00:49
Dissection
03:52
Imaging Vesicle Motility
05:14
Image Analysis
06:21
Results: Lysosome Motility in Neuronal Dendrites
07:21
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
膜运输的调查对于了解神经元功能至关重要。在这里,我们介绍一种量化神经元囊泡运动性的方法。这是一种方便的方法,可以适应于神经系统膜运输的量化。
Tags
Neuron
Vesicle Motility
Membrane Trafficking
Fluorescent Probes
Cell Culture
Cortical Neurons
Endosome
Lysosome
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