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协议侦办主机组织分布，传播模式和效果上浓核病毒的主机健身的棉铃虫
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Immunology and Infection
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JoVE Journal Immunology and Infection
Protocols for Investigating the Host-tissue Distribution, Transmission-mode, and Effect on the Host Fitness of a Densovirus in the Cotton Bollworm
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协议侦办主机组织分布，传播模式和效果上浓核病毒的主机健身的棉铃虫

DOI:
10.3791/55534-v

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11:12 min

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April 12, 2017

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, , , ,
1State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection,Chinese Academy of Agricultural Sciences, 2Tobacco Research Institute,Chinese Academy of Agricultural Sciences, 3Crop and Environment Sciences,Harper Adams University

Chapters

  • 00:05Title
  • 00:40Construction of the HaDV2-free Cotton Bollworm Colony
  • 02:02Preparation of HaDV2-containing Liquid Fluids
  • 03:28Construction of the HaDV2-infected Cotton Bollworm Colony
  • 04:57Tissue Distribution of HaDV2
  • 07:01Bioassays Testing the Effect of HaDV2 on Host Development
  • 09:36Results: Characterization of Effects of Densovirus on Bollworms
  • 10:44Conclusion

Summary

Automatic Translation

Automatic Translation

在这里，我们提出了一个协议，调查宿主组织分布，传输模式和效果上的浓核病毒的宿主健身鳞翅目物种内，棉铃虫。该协议也可以用于研究其他经口传播的病毒和它们的昆虫宿主之间的相互作用。

Tags

Keywords: Cotton BollwormDensovirusHaDV2Virus-host InteractionVirus TransmissionHost FitnessPCRGenetic BackgroundSingle-pair MatingAdult MothsEgg CollectionLarval RearingVirus DetectionVirus-positive StrainVirus-negative Strain

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