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Genetics
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激光局部补骨脂素加合物的单分子分析
JoVE Journal
Genetics
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JoVE Journal
Genetics
Single Molecule Analysis of Laser Localized Psoralen Adducts
Please note that all translations are automatically generated.
Click here for the English version.
激光局部补骨脂素加合物的单分子分析
DOI:
10.3791/55541-v
•
11:46 min
•
April 20, 2017
•
Jing Huang
,
Himabindu Gali
,
Julia Gichimu
,
Marina A. Bellani
,
Durga Pokharel
,
Manikandan Paramasivam
,
Michael M. Seidman
1
Laboratory of Molecular Gerontology
,
National Institute on Aging, National Institutes of Health
Chapters
00:05
Title
00:53
Calibrating the Targeting Laser
04:54
Laser Localized Dig-TMP ICLs
07:44
Harvesting Cells and Stretching DNA Fibers
09:51
Results: Laser Localized Psoralen Adducts Reveal the DNA Damage Response to ICLs
11:21
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
激光器在对DNA损伤的细胞应答的研究经常使用的。然而,它们产生的病变,其间隔,频率,和碰撞与复制叉很少表征。在这里,我们描述了一种方法,使这些参数与激光局部间交联的决心。
Tags
Single Molecule Analysis
Laser Localized Psoralen Adducts
DNA Interstrand Crosslinks
DNA Repair
Laser Activated Compound
Mirror Slide Calibration
Laser Targeting
Biological Calibration
Trimethylpsoralen
GFP Tagged Repair Factor
U2OS Cell Line
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