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档案福尔马林固定，石蜡包埋的人类口咽鳞状细胞癌样品中T细胞亚群的四色荧光免疫组织化学
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Cancer Research
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JoVE Journal Cancer Research
Four-color Fluorescence Immunohistochemistry of T-cell Subpopulations in Archival Formalin-fixed, Paraffin-embedded Human Oropharyngeal Squamous Cell Carcinoma Samples
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档案福尔马林固定，石蜡包埋的人类口咽鳞状细胞癌样品中T细胞亚群的四色荧光免疫组织化学

DOI:
10.3791/55589-v

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07:43 min

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July 29, 2017

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, ,
1Department of Pathology,Leiden University Medical Center, 2Department of Otorhinolaryngology and Head and Neck Surgery,Erasmus University Medical Center, 3Center for Gynecological Oncology Amsterdam,VU Medical Center

Chapters

  • 00:05Title
  • 01:04Antigen Retrieval
  • 02:16Tissue Staining and Analysis
  • 05:12Results: Representative Formalin-fixed, Paraffin-embedded Oropharyngeal Cancer Tissue Staining and Analysis
  • 06:42Conclusion

Summary

Automatic Translation

Automatic Translation

多参数荧光免疫组织化学可用于评估肿瘤微环境中免疫细胞群体的数量，相对分布和定位。该手稿描述了使用这种技术来分析口咽癌中的T细胞亚群。

Tags

ImmunohistochemistryT-cell SubpopulationsOropharyngeal Squamous Cell CarcinomaFormalin-fixed Paraffin-embeddedMulticolorBiomarkersImmune CellsCancer TherapyPatient StratificationImmune Therapies

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