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从成年大鼠心脏同时分离高质量心肌细胞,内皮细胞和成纤维细胞
JoVE Journal
Biology
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JoVE Journal Biology
Simultaneous Isolation of High Quality Cardiomyocytes, Endothelial Cells, and Fibroblasts from an Adult Rat Heart
DOI:

09:38 min

May 19, 2017

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Chapters

  • 00:05Title
  • 00:55Heart Langendorff Perfusion and Digestion
  • 03:43Cardiomyocyte Isolation
  • 05:08Endothelial Cell and Fibroblast Isolation
  • 07:45Results: Representative Cardiac Cell Analyses
  • 08:25Conclusion

Summary

Automatic Translation

已经开发并描述了用于从大鼠心脏分离不同心脏细胞类型的几种方案。在这里,描述了优化的方案,其允许从单一制剂分离高质量的主要心脏细胞类型(心肌细胞,内皮细胞和成纤维细胞),降低了实验成本。

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