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Journal
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Biochemistry
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单分子水平的高精度FRET用于生物分子结构测定
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
High Precision FRET at Single-molecule Level for Biomolecule Structure Determination
Please note that all translations are automatically generated.
Click here for the English version.
单分子水平的高精度FRET用于生物分子结构测定
DOI:
10.3791/55623-v
•
11:24 min
•
May 13, 2017
•
Junyan Ma
,
Inna S. Yanez-Orozco
,
Soheila Rezaei Adariani
,
Drew Dolino
,
Vasanthi Jayaraman
,
Hugo Sanabria
1
Department of Chemistry
,
Clemson University
,
2
Department of Physics and Astronomy
,
Clemson University
,
3
Department of Biochemistry and Molecular Biology, Center for Membrane Biology, Graduate School for Biomedical Sciences
,
University of Texas Health Science Center
Chapters
00:05
Title
01:07
Preparation of Materials
02:57
Pulsed Interleaved Excitation-Multiparameter Fluorescence Detection (PIE-MFD) Instrument Setup
06:14
Burst Integration Fluorescence Lifetime Analysis of Standards
07:34
dsDNA and Protein Sample Measurements and Analysis
09:28
Results: Inter-dye Distance Determination in Single Molecules
10:54
Conclusion
Summary
Automatic Translation
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Automatic Translation
本文介绍了单分子水平高精度FRET实验方案。此外,该方法可用于鉴定N-甲基-D-天冬氨酸(NMDA)受体的配体结合结构域中的三种构象状态。确定精确的距离是建立基于FRET实验的结构模型的第一步。
Tags
High Precision FRET
Single-molecule
Biomolecule Structure Determination
Förster Resonance Energy Transfer
Multiparameter Fluorescence Detection
Interdye Distance Measurement
NMDA Receptor
Conformational States
Structural Model
Dynamic Biomolecules
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