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Bioengineering
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微孔阵列平台中微生物群落发展的组装与跟踪
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Bioengineering
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Bioengineering
Assembly and Tracking of Microbial Community Development within a Microwell Array Platform
Please note that all translations are automatically generated.
Click here for the English version.
微孔阵列平台中微生物群落发展的组装与跟踪
DOI:
10.3791/55701-v
•
09:24 min
•
June 06, 2017
•
Andrea C. Timm
,
Michelle C. Halsted
,
Jared L. Wilmoth
,
Scott T. Retterer
3
1
Biosciences Division
,
Oak Ridge National Laboratory
,
2
Bredesen Center for Interdisciplinary Research and Graduate Education
,
University of Tennessee
,
3
Center for Nanophase Materials Sciences
,
Oak Ridge National Laboratory
Chapters
00:05
Title
00:50
Bacterial Seeding
02:31
Sealing the Wells with an Agarose Coated Coverslip and Imaging
04:53
Bacterial Growth Analysis
07:10
Results: Growth of
P. aeruginosa
Mutants
08:28
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
微生物群落的发展取决于环境结构,成员丰度,特征和相互作用等因素的结合。该协议描述了一种合成的微制造环境,用于同时跟踪包含在femtoliter井中的数千个社区,其中可以近似关键因素,如利基尺寸和约束。
Tags
Microbial Community
Microwell Array
High-throughput
Parallel Analysis
Spatial Constraints
Microbial Interactions
Microbial Ecology
Community Development
Bacterial Culture
BSA
PBS
R2A Medium
Glycerol
Optical Density
Agarose-coated Coverslip
PDMS Spacers
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