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新生儿鼠耳蜗外科手术技术<em>体外</em>听力研究筛选工具

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Medicine

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JoVE Journal Medicine

Neonatal Murine Cochlear Explant Technique as an In Vitro Screening Tool in Hearing Research

Please note that all translations are automatically generated. Click here for the English version.

新生儿鼠耳蜗外科手术技术<em>体外</em>听力研究筛选工具

DOI:

10.3791/55704-v

•

08:30 min

•

June 08, 2017

•

Lukas D. Landegger^2,3, Sonam Dilwali⁴, Konstantina M. Stankovic^2,4

¹Eaton Peabody Laboratories, Department of Otolaryngology,Massachusetts Eye and Ear, ²Department of Otolaryngology,Harvard Medical School, ³Department of Otolaryngology,Vienna General Hospital, Medical University of Vienna, ⁴Harvard Program in Speech and Hearing Bioscience and Technology

Chapters

00:05Title
00:53Macroscopic Dissection of the Temporal Bone
02:06Microscopic Extraction of the Cochleae
04:31Culture of Cochlear Explants
05:59Treatment of Explants
06:38Results: Research Applications of Cochlear Explants
07:58Conclusion

Summary

Automatic Translation

该方案的目的是证明新生儿鼠耳蜗外植体的制备，培养，治疗和免疫染色。该技术可用作听力研究中的体外筛选工具。

Tags

Keywords: Neonatal Murine Cochlear Explant In Vitro Hearing Research Hair Cells Synapses Neurites Neurons Ex Vivo Therapeutic Compounds Screening Tool Dissection Organ Of Corti Spiral Ligament

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