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新生児マウス蝸牛移植法<em>インビトロ</em>聴覚研究のスクリーニングツール
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JoVE Journal
Medicine
Neonatal Murine Cochlear Explant Technique as an
In Vitro
Screening Tool in Hearing Research
Please note that all translations are automatically generated.
Click here for the English version.
新生児マウス蝸牛移植法<em>インビトロ</em>聴覚研究のスクリーニングツール
DOI:
10.3791/55704-v
•
08:30 min
•
June 08, 2017
•
Lukas D. Landegger
2,3
,
Sonam Dilwali
4
,
Konstantina M. Stankovic
2,4
1
Eaton Peabody Laboratories, Department of Otolaryngology
,
Massachusetts Eye and Ear
,
2
Department of Otolaryngology
,
Harvard Medical School
,
3
Department of Otolaryngology
,
Vienna General Hospital, Medical University of Vienna
,
4
Harvard Program in Speech and Hearing Bioscience and Technology
Chapters
00:05
Title
00:53
Macroscopic Dissection of the Temporal Bone
02:06
Microscopic Extraction of the Cochleae
04:31
Culture of Cochlear Explants
05:59
Treatment of Explants
06:38
Results: Research Applications of Cochlear Explants
07:58
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
このプロトコールの目的は、新生仔ネズミの蝸牛外植片の調製、培養、処置および免疫染色を実証することである。この技術は、聴覚研究
におけるインビトロ
スクリーニングツールとして利用することができる。
Tags
Neonatal Murine
Cochlear Explant
In Vitro
Hearing Research
Hair Cells
Synapses
Neurites
Neurons
Ex Vivo
Therapeutic Compounds
Screening Tool
Dissection
Organ Of Corti
Spiral Ligament
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