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Neonatal Murine Cochlear Explant Technique som en<em> In Vitro</em> Screening Tool i Hearing Research
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Neonatal Murine Cochlear Explant Technique as an In Vitro Screening Tool in Hearing Research

Neonatal Murine Cochlear Explant Technique som en<em> In Vitro</em> Screening Tool i Hearing Research

DOI:
10.3791/55704-v

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08:30 min

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June 08, 2017

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, ,
1Eaton Peabody Laboratories, Department of Otolaryngology,Massachusetts Eye and Ear, 2Department of Otolaryngology,Harvard Medical School, 3Department of Otolaryngology,Vienna General Hospital, Medical University of Vienna, 4Harvard Program in Speech and Hearing Bioscience and Technology

Chapters

  • 00:05Title
  • 00:53Macroscopic Dissection of the Temporal Bone
  • 02:06Microscopic Extraction of the Cochleae
  • 04:31Culture of Cochlear Explants
  • 05:59Treatment of Explants
  • 06:38Results: Research Applications of Cochlear Explants
  • 07:58Conclusion

Summary

Automatic Translation

Automatic Translation

Formålet med denne protokol er at demonstrere forberedelse, kultur, behandling og immunostaining af neonatale murine cochleære eksplantater. Teknikken kan udnyttes som et in vitro- screeningsværktøj til høreforskning.

Tags

Keywords: Neonatal MurineCochlear ExplantIn VitroHearing ResearchHair CellsSynapsesNeuritesNeuronsEx VivoTherapeutic CompoundsScreening ToolDissectionOrgan Of CortiSpiral Ligament

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