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JoVE Journal
Biochemistry

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用天然电泳,凝胶电泳和电解法分析线粒体电子传递链的超复合物
 
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用天然电泳,凝胶电泳和电解法分析线粒体电子传递链的超复合物

Article DOI: 10.3791/55738-v 08:37 min June 1st, 2017
June 1st, 2017

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Summary

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该方案描述了使用天然电泳分离功能性线粒体电子传递链复合物(Cx)IV及其超复合物,以揭示其组装和结构信息。天然凝胶可以进行免疫印迹,凝胶内测定,并通过电洗脱纯化以进一步表征单个复合物。

Tags

生物化学,第124期,线粒体,电子传递链,呼吸面罩,合成体,透明天然电泳,凝胶内测定,电洗脱
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