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Journal
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Biochemistry
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用天然电泳,凝胶电泳和电解法分析线粒体电子传递链的超复合物
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Biochemistry
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JoVE Journal
Biochemistry
Analyzing Supercomplexes of the Mitochondrial Electron Transport Chain with Native Electrophoresis, In-gel Assays, and Electroelution
Please note that all translations are automatically generated.
Click here for the English version.
用天然电泳,凝胶电泳和电解法分析线粒体电子传递链的超复合物
DOI:
10.3791/55738-v
•
08:37 min
•
June 01, 2017
•
Gisela Beutner
,
George Arthur Porter Jr.
1
Department of Pediatrics-Division Cardiology
,
University of Rochester
Chapters
00:05
Title
00:58
In-gel Assays for Cx-I and Cx-V
03:19
Electroelution
05:59
Results: Isolation, Visualization, amd Analysis of Mitochondrial Supercomplexes
07:00
Conclusion
Summary
Automatic Translation
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Automatic Translation
该方案描述了使用天然电泳分离功能性线粒体电子传递链复合物(Cx)IV及其超复合物,以揭示其组装和结构信息。天然凝胶可以进行免疫印迹,凝胶内测定,并通过电洗脱纯化以进一步表征单个复合物。
Tags
Mitochondrial Electron Transport Chain
Supercomplexes
Native Electrophoresis
In-gel Assays
Electroelution
Complex-1 Assay
Complex-5 Assay
Detergents
NADH
Oligomycin
Nitro Blue Tetrazolium
Lead Nitrate
ATP
Bioenergetics
Physiological Integrity
Membrane Protein Complexes
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