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瞬时表达和重组蛋白的细胞定位在培养的昆虫细胞
JoVE Journal
Biology
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JoVE Journal Biology
Transient Expression and Cellular Localization of Recombinant Proteins in Cultured Insect Cells
DOI:

12:09 min

April 20, 2017

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Chapters

  • 00:05Title
  • 01:03Insect Cell Culture Maintenance
  • 04:37Insect Cell Transfection
  • 07:20Confocal Fluorescence Microscopy
  • 09:33Results: Protein Localization is Visualized within Live Insect Cells
  • 11:37Conclusion

Summary

Automatic Translation

Nonlytic昆虫细胞表达系统没有得到充分利用进行生产,细胞运输/定位,和重组蛋白质功能分析。这里,我们描述的方法来产生在市售鳞翅目细胞系的表达载体和随后的瞬时表达。与亚细胞荧光标记蛋白烟粉虱水通道蛋白的共定位还提出。

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