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通过显微注射卵母细胞生成转基因小鼠
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Developmental Biology
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JoVE Journal Developmental Biology
Generation of Genetically Modified Mice through the Microinjection of Oocytes
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通过显微注射卵母细胞生成转基因小鼠

DOI:
10.3791/55765-v

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10:19 min

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June 15, 2017

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,
1Transgenic Animal Unit, Mark Wainwright Analytical Centre,University of New South Wales

Chapters

  • 00:05Title
  • 00:53Synthesis of CRISPER Components
  • 04:21Injection Mix and Pronuclear and Cytoplasmic Injections
  • 06:31Reimplantation
  • 08:21Results: Microinjection of Oocytes to Generate Genetically Modified Mice
  • 09:49Conclusion

Summary

Automatic Translation

Automatic Translation

小鼠卵母细胞的显微注射通常用于经典转基因（ 即转基因的随机整合）和CRISPR介导的基因靶向。该协议审查显微注射的最新发展，特别强调质量控制和基因分型策略。

Tags

AI-generated MiceCRISPR-Cas9 Gene EditingOocyte MicroinjectionTransgenic Mouse ProductionRNA SynthesisIn Vitro TranscriptionDNA Purification

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