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通过显微注射卵母细胞生成转基因小鼠
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Generation of Genetically Modified Mice through the Microinjection of Oocytes
DOI:

10:19 min

June 15, 2017

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Chapters

  • 00:05Title
  • 00:53Synthesis of CRISPER Components
  • 04:21Injection Mix and Pronuclear and Cytoplasmic Injections
  • 06:31Reimplantation
  • 08:21Results: Microinjection of Oocytes to Generate Genetically Modified Mice
  • 09:49Conclusion

Summary

Automatic Translation

小鼠卵母细胞的显微注射通常用于经典转基因( 转基因的随机整合)和CRISPR介导的基因靶向。该协议审查显微注射的最新发展,特别强调质量控制和基因分型策略。

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