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Immunology and Infection
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通过流式细胞术分析来自中枢神经系统的小胶质细胞和单核细胞衍生的巨噬细胞
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Analysis of Microglia and Monocyte-derived Macrophages from the Central Nervous System by Flow Cytometry
Please note that all translations are automatically generated.
Click here for the English version.
通过流式细胞术分析来自中枢神经系统的小胶质细胞和单核细胞衍生的巨噬细胞
DOI:
10.3791/55781-v
•
10:43 min
•
June 22, 2017
•
Elodie Martin
2,3
,
Mohamed El-Behi
2,3
,
Bertrand Fontaine
2,3,4
,
Cecile Delarasse
2,3
1
Inserm U 1227, CNRS UMR 7225
,
2
Sorbonne Universités
,
UPMC, University of Paris
,
3
Institut du Cerveau et de la Moelle épinière, ICM
,
4
AP-HP, Hôpital de la Pitié Salpêtrière;
Chapters
00:05
Title
00:52
Perfusion and Brain and Spinal Cord Dissection
03:18
Cell Dissociation and Density Gradient Separation
06:00
Cell Labeling for Flow Cytometry
08:06
Results: Representative Brain and Spinal Cord Macrophage Subpopulation Analysis
09:31
Conclusion
Summary
Automatic Translation
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Automatic Translation
该方案通过流式细胞术提供成年小鼠中枢神经系统中巨噬细胞亚群的分析,有助于研究由这些细胞表达的多种标志物。
Tags
Microglia
Monocyte-derived Macrophages
Central Nervous System
Flow Cytometry
Neuroimmunology
Macrophage Subpopulations
Cell Marker Analysis
Cell Isolation
Spinal Cord
Brain
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