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Isolation of Primary Murine Retinal Ganglion Cells (RGCs) by Flow Cytometry
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1Department of Ophthalmology, Hamilton Eye Institute,University of Tennessee Health Science Center, 2Department of Microbiology, Immunology and Biochemistry,University of Tennessee Health Science Center, 3Department of Anatomy and Neurobiology,University of Tennessee Health Science Center, 4Department of Pharmaceutical Sciences,University of Tennessee Health Science Center
Chapters
- 00:05Title
- 02:07Retinal Suspension Preparation
- 04:38Retinal Cell Immonolabeling and Sorting
- 08:21Results: Representative Retinal Ganglion Cell (RGC) Analyses
- 09:44Conclusion
Millions of people suffer from retinal degenerative diseases that result in irreversible blindness. A common element of many of these diseases is the loss of retinal ganglion cells (RGCs). This detailed protocol describes the isolation of primary murine RGCs by positive and negative selection with flow cytometry.
