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Journal
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Bioengineering
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通过流式细胞术分离原代小鼠视网膜神经节细胞(RGC)
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
Isolation of Primary Murine Retinal Ganglion Cells (RGCs) by Flow Cytometry
Please note that all translations are automatically generated.
Click here for the English version.
通过流式细胞术分离原代小鼠视网膜神经节细胞(RGC)
DOI:
10.3791/55785-v
•
11:01 min
•
July 05, 2017
•
Sumana R. Chintalapudi
,
Need N. Patel
,
Zachary K. Goldsmith
,
Levon Djenderedjian
,
Xiang Di Wang
,
Tony N. Marion
,
Monica M. Jablonski
3,4
,
Vanessa M. Morales-Tirado
2
1
Department of Ophthalmology, Hamilton Eye Institute
,
University of Tennessee Health Science Center
,
2
Department of Microbiology, Immunology and Biochemistry
,
University of Tennessee Health Science Center
,
3
Department of Anatomy and Neurobiology
,
University of Tennessee Health Science Center
,
4
Department of Pharmaceutical Sciences
,
University of Tennessee Health Science Center
Chapters
00:05
Title
02:07
Retinal Suspension Preparation
04:38
Retinal Cell Immonolabeling and Sorting
08:21
Results: Representative Retinal Ganglion Cell (RGC) Analyses
09:44
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
数百万人患有导致不可逆失明的视网膜退行性疾病。许多这些疾病的常见因素是视网膜神经节细胞(RGC)的丧失。该详细方案描述了通过流式细胞术的阳性和阴性选择分离原代鼠RGC。
Tags
Primary Murine Retinal Ganglion Cells
RGCs
Flow Cytometry
Retinal Degeneration
Retinal Ganglion Cell Isolation
Cell Sorting
Eye Enucleation
Optic Nerve
Cell Culture
Cell Line
Vision Research
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