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通过流式细胞术分离原代小鼠视网膜神经节细胞(RGC)
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Isolation of Primary Murine Retinal Ganglion Cells (RGCs) by Flow Cytometry
DOI:

11:01 min

July 05, 2017

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Chapters

  • 00:05Title
  • 02:07Retinal Suspension Preparation
  • 04:38Retinal Cell Immonolabeling and Sorting
  • 08:21Results: Representative Retinal Ganglion Cell (RGC) Analyses
  • 09:44Conclusion

Summary

Automatic Translation

数百万人患有导致不可逆失明的视网膜退行性疾病。许多这些疾病的常见因素是视网膜神经节细胞(RGC)的丧失。该详细方案描述了通过流式细胞术的阳性和阴性选择分离原代鼠RGC。

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