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Isolering af primære murin retinalganglionceller (RGC'er) ved flowcytometri
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1Department of Ophthalmology, Hamilton Eye Institute,University of Tennessee Health Science Center, 2Department of Microbiology, Immunology and Biochemistry,University of Tennessee Health Science Center, 3Department of Anatomy and Neurobiology,University of Tennessee Health Science Center, 4Department of Pharmaceutical Sciences,University of Tennessee Health Science Center
Chapters
- 00:05Title
- 02:07Retinal Suspension Preparation
- 04:38Retinal Cell Immonolabeling and Sorting
- 08:21Results: Representative Retinal Ganglion Cell (RGC) Analyses
- 09:44Conclusion
Millioner af mennesker lider af retinal degenerative sygdomme, der resulterer i irreversibel blindhed. Et fælles element i mange af disse sygdomme er tabet af retinale ganglionceller (RGC'er). Denne detaljerede protokol beskriver isoleringen af primære murine RGC'er ved positiv og negativ selektion med flowcytometri.
