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Live-cell mätning av luktämnet Receptor aktiveringen med en realtid cAMP-analys
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Live-cell Measurement of Odorant Receptor Activation Using a Real-time cAMP Assay
DOI:

09:11 min

October 02, 2017

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Chapters

  • 00:05Title
  • 00:50Plating and Transfection of Hana3A Cells
  • 03:03Transfection of Plasmids
  • 05:24Stimulation and Measuring OR Activity Using the Real-time cAMP Assay
  • 07:27Results: Kinetic Measurements of the Response of OR5AN1 to Muscone
  • 08:23Conclusion

Summary

Automatic Translation

Karaktärisera funktionen av luktämnet receptorer serverar en oumbärlig del i processen deorphanization. Vi beskriver en metod att mäta aktivering av luktämnet receptorer i realtid med en cAMP-analys.

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