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一种快速高效的解剖蛹翅的方法, 适用于 Immunodetections 或 PCR 检测
JoVE Journal
Developmental Biology
Author Produced
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JoVE Journal Developmental Biology
A Rapid and Efficient Method to Dissect Pupal Wings of Drosophila Suitable for Immunodetections or PCR Assays
DOI:

07:11 min

December 30, 2017

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Chapters

  • 00:00Title
  • 00:30Dissection Overview
  • 02:23Dissection of pupal wings
  • 04:38Multiple pupae dissection
  • 05:32Antibody stained pupal wings and PCR
  • 06:23Conclusions

Summary

Automatic Translation

果蝇组织中准确检测转录和蛋白质的能力对于研究它们与发展过程有关的丰度和定位是至关重要的。这里是一个简单的过程解剖蛹翅膀的描述。这些翅膀可以作为样品在一些技术 (免疫组织化学, PCR 检测,)。

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