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Developmental Biology
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一种快速高效的解剖蛹翅的方法, 适用于 Immunodetections 或 PCR 检测
JoVE Journal
Developmental Biology
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JoVE Journal
Developmental Biology
A Rapid and Efficient Method to Dissect Pupal Wings of
Drosophila
Suitable for Immunodetections or PCR Assays
Please note that all translations are automatically generated.
Click here for the English version.
一种快速高效的解剖蛹翅的方法, 适用于 Immunodetections 或 PCR 检测
DOI:
10.3791/55854-v
•
07:11 min
•
December 30, 2017
•
Carmen Bolatto
,
Cristina Parada
,
Victoria Colmenares
1
Departamento de Histología y Embriología, Facultad de Medicina
,
Laboratorio de Biología del Desarrollo
Chapters
00:00
Title
00:30
Dissection Overview
02:23
Dissection of pupal wings
04:38
Multiple pupae dissection
05:32
Antibody stained pupal wings and PCR
06:23
Conclusions
Summary
Automatic Translation
English (Original)
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français (French)
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italiano (Italian)
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Automatic Translation
在
果蝇
组织中准确检测转录和蛋白质的能力对于研究它们与发展过程有关的丰度和定位是至关重要的。这里是一个简单的过程解剖蛹翅膀的描述。这些翅膀可以作为样品在一些技术 (免疫组织化学, PCR 检测,
等
)。
Tags
Drosophila
Pupal Wings
Immunodetection
PCR Assays
Rapid Dissection
Efficient Method
Zero-hour APF Pupa
Developmental Time
Pupal Case
Forceps
Double-sided Tape
Ventral Side
Operculum
Cuticle
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