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Chapters
- 00:05Title
- 00:44Cultivation of HeLa-derived Cell Lines
- 01:48Cell Transfection
- 03:21Induction of Local DNA Lesions and Confocal Microscopy
- 04:47Fluorescence Lifetime Image Microscopy
- 06:24FRET Efficiency Using the FLIM-FRET Script
- 07:48FRAP Analysis
- 08:50Results: Analysis of Recruitment of mCherry-tagged PCNA and -53BP1 at DNA Lesions in HeLa Cells
- 11:00Conclusion
