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应用一种适应微流控嗅觉芯片的神经活性成像对雄性线虫神经元的信息素反应

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Using an Adapted Microfluidic Olfactory Chip for the Imaging of Neuronal Activity in Response to Pheromones in Male C. Elegans Head Neurons

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应用一种适应微流控嗅觉芯片的神经活性成像对雄性线虫神经元的信息素反应

DOI:

10.3791/56026-v

•

07:31 min

•

September 07, 2017

•

Douglas K. Reilly, Daniel E. Lawler, Dirk R. Albrecht², Jagan Srinivasan

¹Department of Biology and Biotechnology,Worcester Polytechnic Institute, ²Department of Biomedical Engineering,Worcester Polytechnic Institute

Chapters

00:05Title
00:41Microfluidic Device Set Up
02:54Loading C. elegans into the Microfluidic Device
04:44Olfactory Stimulation of C. elegans Using a Microfluidic Device
05:43Results: Neural Response to Chemical Stimuli
06:45Conclusion

Summary

Automatic Translation

在这里描述了使用一个适应的 "嗅觉芯片" 的有效钙显像的C. 线虫男性。还显示了男性接触甘油和信息素的研究。

Tags

Microfluidic Olfactory C. Elegans Pheromones Calcium Transience Neuronal Activity Sex-specific Neural Circuit Biogenic Pheromones Fluorescin S Basal Micro-fluidic Device Flow Control Vacuum GFP Filter NGM Agar Plate

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