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Fourier-Based Diffraction Analysis of Live Caenorhabditis elegans
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Engineering
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JoVE Journal Engineering
Fourier-Based Diffraction Analysis of Live Caenorhabditis elegans

Fourier-Based Diffraction Analysis of Live Caenorhabditis elegans

DOI:
10.3791/56154-v

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08:24 min

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September 13, 2017

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, , , , ,
1Physics and Astronomy Department,Vassar College, 2Division of Science, Mathematics and Computing,Bard College at Simon’s Rock, 3Biology Department,Vassar College

Chapters

  • 00:05Title
  • 01:05Optical Setup
  • 02:47Nematode and Cuvette Preparation
  • 04:13Real Time Data-Aquisition of Diffraction Pattern Intensity
  • 05:59Results: Power Spectra of Roller and Wild Type Fraunhofer Diffraction Series
  • 07:15Conclusion

Summary

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Automatic Translation

This manuscript describes how to distinguish different nematodes using far-field diffraction signatures. We compare the locomotion of 139 wild type and 108 "Roller" C. elegans by averaging frequencies associated with the temporal Fraunhofer diffraction signature at a single location using a continuous wave laser.

Tags

Keywords: Fourier-based Diffraction AnalysisCaenorhabditis ElegansLocomotionNeurobiologyMicro OrganismsHelium Neon LaserNeutral Density FilterPeriscopeCuvettePhoto DiodeDigital OscilloscopeNematodesNGM Agar

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