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Developmental Biology
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マウス着床前胚幹細胞の誘導
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Developmental Biology
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JoVE Journal
Developmental Biology
Derivation of Stem Cell Lines from Mouse Preimplantation Embryos
Please note that all translations are automatically generated.
Click here for the English version.
マウス着床前胚幹細胞の誘導
DOI:
10.3791/56171-v
•
12:59 min
•
August 20, 2017
•
Marta Vila-Cejudo
,
Elena Ibañez
,
Josep Santalo
1
Unitat de Biologia Cellular, Facultat de Biociències
,
Universitat Autònoma de Barcelona
Chapters
00:00
Title
01:08
Feeder Cells Inctivation and Storage
03:21
Feeder Cells Culture
05:14
Embryo Collection and Culture
06:32
ESC Derivation
09:06
ESC Culture and Maintenance
11:22
Results: Validation of the Stemness and the
In Vitro
Differentiation Potential of the ESC Lines Established
12:07
Conclusion
Summary
Automatic Translation
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Automatic Translation
この資料では、効率的に派生し、胚盤胞の段階でマウス胚からの多能性幹細胞株を培養するプロトコルについて説明します。
Tags
Stem Cells
Embryonic Stem Cells
Mouse Embryos
Preimplantation Embryos
Cell Derivation
Feeder Cells
Fibroblasts
Mitomycin C
Inactivation
Cell Culture
Cell Counting
Cryopreservation
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