Genetics
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Una metodología estándar para examinar in situ mutagenicidad en función de la mutación de punto reparación catalizada por CRISPR/Cas9 y SsODN en células humanas
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Summary August 25th, 2017
Please note that all translations are automatically generated.
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Este protocolo describe el flujo de trabajo de un gen CRISPR/Cas9 basado en sistema para la reparación de las mutaciones de punto en células de mamíferos. Aquí, utilizamos un enfoque combinatorio gene edición con una detallado seguimiento estrategia experimental para medir la formación de indel en el sitio de destino, en esencia, analizar in situ la mutagénesis.
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