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Journal
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Genetics
/
激光 Microirradiation 研究
体内
细胞对简单复杂 DNA 损伤的反应
JoVE Journal
Genetics
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JoVE Journal
Genetics
Laser Microirradiation to Study
In Vivo
Cellular Responses to Simple and Complex DNA Damage
Please note that all translations are automatically generated.
Click here for the English version.
激光 Microirradiation 研究
体内
细胞对简单复杂 DNA 损伤的反应
DOI:
10.3791/56213-v
•
10:44 min
•
January 31, 2018
•
Xiangduo Kong*
1
,
Gladys M.S. Cruz*
2
,
Bárbara A. Silva
,
Nicole M. Wakida
,
Nima Khatibzadeh
,
Michael W. Berns
3,4
,
Kyoko Yokomori
1
Department of Biological Chemistry, School of Medicine
,
University of California, Irvine
,
2
Beckman Laser Institute and Medical Clinic
,
University of California, Irvine
,
3
Department of Developmental and Cell Biology, School of Biological Sciences
,
University of California, Irvine
,
4
Department of Biomedical Engineering and Surgery
,
University of California, Irvine
Chapters
00:05
Title
01:25
Transient Transfection
04:09
Titration of Laser Input Power
05:40
Quantitative Fluorescence Analysis of Immunostained or Live Cells
06:54
Cell Cycle and G1 Phase Synchronization
07:46
Results:
In Vivo
Cellular Responses to Complex DNA Damage
09:27
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议的目的是描述如何使用激光 microirradiation 诱导不同类型的 dna 损伤, 包括相对简单的断链和复杂的损害, 研究 dna 损伤信号和修复因子在损伤部位的组装
在体内
.
Tags
Laser Microirradiation
DNA Damage
DNA Repair
DNA Repair Factors
Cellular Response
Real-time Analysis
Single-cell Analysis
Fluorescently-tagged Proteins
53BP1
NTH1
Liposome-mediated Transfection
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