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Produksjon av genmodifiserte Golden syrer Hamsters ved Pronuclear injeksjon av CRISPR/Cas9 komplekset
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1Department of Animal, Dairy, and Veterinary Sciences,Utah State University, 2National Centre for International Research in Cell and Gene Therapy, Sino-British Research Centre, School of Basic Medical Sciences,Zhengzhou University, 3Department of Animal Science Division of Applied Life Science (BK21 Plus),Gyeongsang National University, 4Institute of Agriculture and Life Science,Gyeongsang National University, 5Centre for Molecular Oncology, Barts Cancer Institute,Queen Mary University of London
Chapters
- 00:05Title
- 01:06Zygote Isolation
- 03:31PN Injection
- 04:52Results: Evaluation of Gene Targeting in Golden Syrian Hamster by the Cas9/sgRNA System
- 05:36Conclusion
Pronuclear (PN) injeksjon av de klynget regelmessig interspaced kort palindromic gjentar (CRISPR) og CRISPR-assosiert protein-9 nuclease (CRISPR/Cas9) er en svært effektiv metode for å produsere genmodifiserte golden syrer hamsters. Her beskriver vi detaljerte PN injeksjon protokollen for produksjon av genet knockout hamstere med CRISPR/Cas9-systemet.
