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慢性炎症状态个体的单核细胞轮回和泡沫形成的定量研究
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Immunology and Infection
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JoVE Journal Immunology and Infection
Quantification of Monocyte Transmigration and Foam Cell Formation from Individuals with Chronic Inflammatory Conditions
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慢性炎症状态个体的单核细胞轮回和泡沫形成的定量研究

DOI:
10.3791/56293-v

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09:41 min

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October 17, 2017

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, , , ,
1Centre for Biomedical Research,Burnet Institute, 2School of Health and Biomedical Sciences,RMIT University, 3Department of Infectious Diseases,Monash University, 4University of California, Los Angeles

Chapters

  • 00:05Title
  • 00:50Culturing HUVEC Monolayer on Collagen Gels
  • 03:38Activation of HUVEC Monolayer and Isolation/Activation of Monocytes for Transmigration
  • 05:44Quantification of Foam Cells and Macrophages by Microscopy or Flow Cytometry
  • 07:42Results: Characterizing the Transmigrating Mice
  • 09:09Conclusion

Summary

Automatic Translation

Automatic Translation

我们描述了一项协议, 以测量单核细胞在人的内皮单分子膜的轮回, 并随后成熟成泡沫电池。这提供了一种通用的方法来评估的动脉粥样硬化性质的人单核细胞分离的不同疾病的情况下, 并评估血液中的因素, 可能会提高这种倾向。

Tags

Monocyte TransmigrationFoam Cell FormationChronic Inflammatory ConditionsCardiovascular DiseaseAtherosclerosisEndothelial CellsCell CultureCollagen GelHUVECMicroscopyFlow Cytometry

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