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Journal
/
Genetics
/
无基因克隆的基因破坏的
变形链球菌
菌株的生成
JoVE Journal
Genetics
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JoVE Journal
Genetics
Generation of a Gene-disrupted
Streptococcus mutans
Strain Without Gene Cloning
Please note that all translations are automatically generated.
Click here for the English version.
无基因克隆的基因破坏的
变形链球菌
菌株的生成
DOI:
10.3791/56319-v
•
12:06 min
•
October 23, 2017
•
Takatoshi Murata
,
Ayako Okada
,
Khairul Matin
2,3
,
Nobuhiro Hanada
1
Department of Translational Research
,
Tsurumi University School of Dental Medicine
,
2
Endowed Department of International Oral Health Science (affiliated with Department of Translational Research)
,
Tsurumi University School of Dental Medicine
,
3
Cariology and Operative Dentistry, Graduate School of Medical and Dental Sciences
,
Tokyo Medical and Dental University
Chapters
00:05
Title
01:14
Genomic DNA Extractions from
S. mutans
04:01
PCR Amplification
06:43
Cell Transformation
07:43
Verification of Gene Disruption
08:41
Phenotypic Analysis
10:16
Results: 2-Step Fusion PCR Generates
S. mutans
Strains with Gene Disruptions
11:26
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们描述了一种简便, 快速, 和相对廉价的方法, 产生基因中断的
变形链球菌
菌株;这种技术可以适应于各种物种的基因中断菌株的产生。
Tags
Streptococcus Mutans
Gene Disruption
Two-step Fusion PCR
Electroporation
Nested Primers
Genomic DNA Extraction
Bead-beating
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