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遺伝子破壊ミュータンス連鎖球菌緊張せず遺伝子クローニングの生成
JoVE Journal
Genetics
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JoVE Journal Genetics
Generation of a Gene-disrupted Streptococcus mutans Strain Without Gene Cloning
DOI:

12:06 min

October 23, 2017

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Chapters

  • 00:05Title
  • 01:14Genomic DNA Extractions from S. mutans
  • 04:01PCR Amplification
  • 06:43Cell Transformation
  • 07:43Verification of Gene Disruption
  • 08:41Phenotypic Analysis
  • 10:16Results: 2-Step Fusion PCR Generates S. mutans Strains with Gene Disruptions
  • 11:26Conclusion

Summary

Automatic Translation

遺伝子破壊ミュータンス連鎖球菌の世代の簡易・迅速かつ比較的安価な手法について述べる株;この手法は、様々 な種の遺伝子破壊系統の世代の適応可能性があります。

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