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Journal
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Cancer Research
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单细胞下游特征的靶细胞预富集与全基因组扩增
JoVE Journal
Cancer Research
This content is Free Access.
JoVE Journal
Cancer Research
Target Cell Pre-enrichment and Whole Genome Amplification for Single Cell Downstream Characterization
Please note that all translations are automatically generated.
Click here for the English version.
单细胞下游特征的靶细胞预富集与全基因组扩增
DOI:
10.3791/56394-v
•
10:12 min
•
May 15, 2018
•
Shukun Chen*
1
,
Amin El-Heliebi*
1
,
Julia Schmid
,
Karl Kashofer
,
Zbigniew T. Czyż
,
Bernhard Michael Polzer
,
Klaus Pantel
,
Thomas Kroneis
5
,
Peter Sedlmayr
1
Institute of Cell Biology, Histology and Embryology
,
Medical University of Graz
,
2
Institute of Pathology
,
Medical University of Graz
,
3
Fraunhofer Institute for Toxicology and Experimental Medicine ITEM
,
4
Department of Tumor Biology
,
University Medical Center Hamburg-Eppendorf
,
5
Sahlgrenska Cancer Center
,
University of Gothenburg
Chapters
00:05
Title
01:28
Preparation of Target Cells
02:41
Charging the Wire
03:27
Counting Cells on the Wire
04:03
Detachment and Recovery of Target Cells
05:25
Single-cell Sampling Using Micromanipulation
06:37
Single-cell Sampling Using Laser Microdissection
07:47
Results: Use of Functionalized Wire for
Ex Vivo
Characterization and Genomic Analysis at Single Cell Level
08:59
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议是从非目标背景细胞的混合物中恢复和制备稀有靶细胞, 用于单细胞水平的分子遗传表征。DNA 质量等于未处理的单个细胞, 并允许单细胞应用 (筛查和靶向分析)。
Tags
Single Cell
Cell Suspension
Liquid Biopsy
Cellular Heterogeneity
Circulating Tumor Cells
Cell Characterization
Cell Isolation
Cell Harvesting
Whole Genome Amplification
Pre-enrichment
Hoechst 33342
CFSE Labeling
Hemocytometer
Fluorescence Microscopy
Cell Counting
Cell Resuspension
Peripheral Blood
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