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Journal
/
Developmental Biology
/
人心脏组织对细胞外基质自组装水凝胶的处理
体外
和
体内
应用
JoVE Journal
Developmental Biology
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JoVE Journal
Developmental Biology
Processing of Human Cardiac Tissue Toward Extracellular Matrix Self-assembling Hydrogel for
In Vitro
and
In Vivo
Applications
Please note that all translations are automatically generated.
Click here for the English version.
人心脏组织对细胞外基质自组装水凝胶的处理
体外
和
体内
应用
DOI:
10.3791/56419-v
•
08:41 min
•
December 04, 2017
•
Matthias Becker
2
,
Janita A. Maring
2
,
Barbara Oberwallner
,
Benjamin Kappler
,
Oliver Klein
2
,
Volkmar Falk
3,4,5
,
Christof Stamm
3,4
1
Charité – Universitätsmedizin Berlin
,
corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health
,
2
Berlin-Brandenburg Center for Regenerative Therapies (BCRT)
,
3
German Center for Cardiovascular Research (DZHK)
,
4
Deutsches Herzzentrum Berlin (DHZB)
,
5
Department of Cardiovascular Surgery, Charité – Universitätsmedizin Berlin
,
corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health
Chapters
00:05
Title
00:43
Sectioning Human Myocardium into Slices
02:27
Decellularization of Human Myocardium Slices
04:20
Processing the Decellularized cECM to a Powder
05:50
Enzyme-based Homogenization of cECM Micro-particles
06:56
Results: Effective Removal of Cellular Material Enhances Analysis
08:10
Conclusion
Summary
Automatic Translation
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Automatic Translation
该协议描述了人类心肌的完整细胞, 同时保留了其胞外基质成分。细胞外基质的进一步处理导致微粒和细胞自组装水凝胶的生产。
Tags
Cardiac Extracellular Matrix
Hydrogel
Cell-based Assays
Myocardial Infarction
Cryosectioning
Decellularization
SDS
Article
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