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Journal
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Biochemistry
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植物甘露聚糖细胞壁多糖的结构表征
JoVE Journal
Biochemistry
This content is Free Access.
JoVE Journal
Biochemistry
Structural Characterization of Mannan Cell Wall Polysaccharides in Plants Using PACE
Please note that all translations are automatically generated.
Click here for the English version.
植物甘露聚糖细胞壁多糖的结构表征
DOI:
10.3791/56424-v
•
11:06 min
•
October 16, 2017
•
Venkataramana R. Pidatala*
1,2
,
Amir Mahboubi*
1,2
,
Jenny C. Mortimer
2
1
Joint BioEnergy Institute
,
2
Environmental and Systems Biology, BioSciences Division
,
Lawrence Berkeley National Laboratory
Chapters
00:05
Title
00:37
Aliquoting and Pre-treatment of Alcohol Insoluble Residue (AIR)
02:11
Hydrolysis of AIR with Glycosyl Hydrolases
03:20
Derivitization of Oligosaccharides
04:29
Preparation of PACE Gels
06:33
Running a PACE Gel
07:51
Visualizing a PACE Gel
09:07
Results: Characterization of Plant Cell Wall Glucomannam Structure
10:30
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
介绍了以甘露聚糖为例, 用凝胶电泳 (佩斯) 对多糖进行结构分析的协议。
Tags
Plant Cell Wall
Polysaccharides
Mannan
PACE
Structural Characterization
AIR
Mannanase
ANTS Derivatization
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