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Strukturell karaktärisering av Mannan cellväggen polysackarider i anläggningar som använder takt
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Structural Characterization of Mannan Cell Wall Polysaccharides in Plants Using PACE
DOI:

11:06 min

October 16, 2017

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Chapters

  • 00:05Title
  • 00:37Aliquoting and Pre-treatment of Alcohol Insoluble Residue (AIR)
  • 02:11Hydrolysis of AIR with Glycosyl Hydrolases
  • 03:20Derivitization of Oligosaccharides
  • 04:29Preparation of PACE Gels
  • 06:33Running a PACE Gel
  • 07:51Visualizing a PACE Gel
  • 09:07Results: Characterization of Plant Cell Wall Glucomannam Structure
  • 10:30Conclusion

Summary

Automatic Translation

Ett protokoll för den strukturella analysen av polysackarider med gelelektrofores (PACE), med mannan som exempel, beskrivs.

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