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1School of Public Health of Southeast University,Laboratory of Environment and Biosafety Research Institute of Southeast University in Suzhou, 2Key Laboratory of Child Development and Learning Science (Ministry of Education), School of Biological Science & Medical Engineering,Southeast University, 3School of Public Health,Tianjin Medical University, 4British Columbia Academy,Nanjing Foreign Language School
Chapters
- 00:04Title
- 01:27Preparation of the Columns and Solutions Needed for the Extraction and Determination of Catecholamines
- 04:44Preparation of Real Urine Samples and Mobile Phase
- 06:25PFSPE Extraction and HPLC Analysis
- 09:58Results: Identification and Quantification of Extracted Catecholamines and their Metabolites in Urine
- 11:43Conclusion
Vi presentiamo una comoda estrazione in fase solida accoppiata alla cromatografia liquida (HPLC) con rivelazione elettrochimica (ECD) per la determinazione simultanea di tre neurotrasmettitori di monoammina e due dei loro metaboliti nelle urine dei bambini. Identifichiamo anche il metabolita MHPG come potenziale biomarcatore per la diagnosi precoce del danno cerebrale per neonati.
