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用高压液相色谱法提取和分析儿茶酚胺神经递质及其代谢物的简便方法
JoVE Journal
Chemistry
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JoVE Journal Chemistry
A Convenient Method for Extraction and Analysis with High-Pressure Liquid Chromatography of Catecholamine Neurotransmitters and Their Metabolites
DOI:

13:35 min

March 01, 2018

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Chapters

  • 00:04Title
  • 01:27Preparation of the Columns and Solutions Needed for the Extraction and Determination of Catecholamines
  • 04:44Preparation of Real Urine Samples and Mobile Phase
  • 06:25PFSPE Extraction and HPLC Analysis
  • 09:58Results: Identification and Quantification of Extracted Catecholamines and their Metabolites in Urine
  • 11:43Conclusion

Summary

Automatic Translation

我们提出了一种方便的固相萃取与高压液相色谱 (HPLC) 结合电化学检测 (幼儿发展) 同时测定三单胺类神经递质和两个代谢物在婴儿的尿液中。我们还确定代谢物 MHPG 作为早期诊断婴儿脑损伤的潜在生物标志物。

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