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1School of Public Health of Southeast University,Laboratory of Environment and Biosafety Research Institute of Southeast University in Suzhou, 2Key Laboratory of Child Development and Learning Science (Ministry of Education), School of Biological Science & Medical Engineering,Southeast University, 3School of Public Health,Tianjin Medical University, 4British Columbia Academy,Nanjing Foreign Language School
Chapters
- 00:04Title
- 01:27Preparation of the Columns and Solutions Needed for the Extraction and Determination of Catecholamines
- 04:44Preparation of Real Urine Samples and Mobile Phase
- 06:25PFSPE Extraction and HPLC Analysis
- 09:58Results: Identification and Quantification of Extracted Catecholamines and their Metabolites in Urine
- 11:43Conclusion
Apresentamos uma conveniente extração de fase sólida acoplada a cromatografia líquida de alta pressão (HPLC) com deteção eletroquímica (ECD) para a determinação simultânea de três neurotransmissores monoamina e dois dos seus metabolitos na urina dos bebês. Também identificamos o metabólito MHPG como um potencial biomarcador para o diagnóstico precoce de lesões cerebrais para infantes.
