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Developmental Biology
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用小鼠模型对精子发生细胞学分析的精管壁球技术
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JoVE Journal
Developmental Biology
A Seminiferous Tubule Squash Technique for the Cytological Analysis of Spermatogenesis Using the Mouse Model
Please note that all translations are automatically generated.
Click here for the English version.
用小鼠模型对精子发生细胞学分析的精管壁球技术
DOI:
10.3791/56453-v
•
09:40 min
•
February 06, 2018
•
Stephen R Wellard*
1
,
Jessica Hopkins*
1
,
Philip W. Jordan
1
Department of Biochemistry and Molecular Biology
,
Johns Hopkins Bloomberg School of Public Health
Chapters
00:04
Title
01:12
Dissection and Preparation of Mouse Seminiferous Tubules
03:16
Squashing and Labeling Prepared Seminiferous Tubules
05:47
Analysis and Imaging Tubule Squash Preparations
07:03
Results: Visualization of Meiotic Progression Using the Tubule Squash Preparation
08:35
Conclusion
Summary
Automatic Translation
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Automatic Translation
这个小管壁技术的目的是快速评估发育中鼠精的细胞学特征, 同时保持细胞完整性。这种方法允许研究精子发生的所有阶段, 并可以很容易地实现与其他生物化学和分子生物学方法的研究小鼠减数分裂。
Tags
Seminiferous Tubule
Cytological Analysis
Spermatogenesis
Mouse Model
Spermatogonia
Spermatocytes
Spermatids
Meiosis
Male Reproductive Biology
Spermatogonial Stem Cell Differentiation
Meiotic Chromosome Segregation
Post-meiotic Differentiation
Cellular Integrity
Infertility
Mammalian Systems
Squash Technique
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Fixative Lysis Solution
Poly-L-lysine Coated Glass Slide
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