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斑马鱼幼虫显微注射和成像的微装置
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Developmental Biology
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JoVE Journal Developmental Biology
Microstructured Devices for Optimized Microinjection and Imaging of Zebrafish Larvae
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斑马鱼幼虫显微注射和成像的微装置

DOI:
10.3791/56498-v

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07:40 min

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December 08, 2017

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,
1BioMEMS Resource Center, Department of Surgery,Massachusetts General Hospital–Harvard Medical School–Shriners Burns Hospital

Chapters

  • 00:05Title
  • 01:06Orientation of Zebrafish on Divot Arrays and Microstructured Channels
  • 02:39Microinjection of Zebrafish
  • 04:20Screening of Embryos Using the PDMS Imaging Device
  • 05:45Results: Microinjection and Imagingof Zebrafish Larvae Using Microstructured Devices
  • 07:04Conclusion

Summary

Automatic Translation

Automatic Translation

斑马鱼胚胎和幼虫的显微注射是许多斑马鱼模型中的关键但具有挑战性的技术。在这里, 我们提出了一系列的微型工具, 以帮助稳定和定位斑马鱼的显微注射和成像。

Tags

MicroinjectionZebrafish LarvaeMicrostructured DevicesPDMSMicroarrayMicrofeaturesImmobilizationOrientationImagingSingle-cell ResolutionDisease Models

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