01:17Isolation of Human Peripheral Blood Mononuclear Cells
02:00Tetramer-Associated Magnetic Enrichment of Antigen Specific B-Cells
03:58Staining of Antigen Specific Human B-Cells and Cell Sorting
04:56Single Cell RT-PCR
06:00Screening and Verification of Heavy and Light Chain Expressing Vectors
07:45Production of Monoclonal Antibodies
10:33Results: Detection of Relevant Antigen Specific B-Cells and Generation of Highly Discriminative Monoclonal Antibody from Donor Peripheral Blood Mononuclear Cells
Vi beskriver en metode til produktion af humane antistoffer specifikke for et antigen af interesse, med udgangspunkt i sjældne B celler cirkulerer i humant blod. Generation af disse naturlige antistoffer er effektiv og hurtig, og antistofferne fremstillet kan forskelsbehandle stærkt relaterede antigener.