A subscription to JoVE is required to view this content. Sign in or start your free trial.
1Department of Radiation Biology, Institute of Aerospace Medicine, Space Microbiology Research Group,German Aerospace Center (DLR e.V.), 2Institute of Electrical Engineering and Plasma Technology, Faculty of Electrical Engineering and Information Technology,Ruhr-University Bochum, 3Institute of Electrical Engineering and Plasma Technology, Faculty of Electrical Engineering and Information Technology, Biomedical Applications of Plasma Technology,Ruhr-University Bochum, 4Advanced Light and Electron Microscopy (ZBS 4),Robert Koch Institute
Chapters
- 00:05Title
- 00:55Bacillus subtilis Spore Production and Purification and Sample Preparation of Aerosol-deposited Bacillus subtilis Spores
- 03:03Low Pressure Plasma Treatment and Recovery and Evaluation of Spore Survival
- 05:10Live Cell Microscopy, Tracking of DNA Repair Processes in Germinating Spores, and Scanning Electron Microscopy
- 07:27Results: Bacillus subtilis Spore Survival After Low Pressure Plasma Sterilization
- 09:02Conclusion
Этот протокол иллюстрирует важный подряд шаги, необходимые для оценки релевантности мониторинга жизнеспособность параметров и процессов репарации ДНК в оживлении спор Bacillus subtilis после лечения с плазмой низкого давления путем отслеживания помечены флуоресцентным ДНК ремонт белков через время решена confocal микроскопии и растровая электронная микроскопия.
